FITC标记的原癌基因WIP1抗体
产品名称： FITC标记的原癌基因WIP1抗体
英文名称： Anti-PPM1D/FITC
产品编号： HZ-2447R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-PPM1D/FITC Conjugated antibody
FITC标记的原癌基因WIP1抗体
| 英文名称 | Anti-PPM1D/FITC |
| 中文名称 | FITC标记的原癌基因WIP1抗体 |
| 别 名 | WPP-DOMAIN INTERACTING PROTEIN 1; EC 3.1.3.16; p53 induced protein phosphatase 1; PP2C delta; PP2CD; protein phosphatase 1D magnesium-dependent delta isoform; Protein phosphatase 2C delta isoform; Protein phosphatase 2C isoform delta; Protein phosphatase magnesium dependent 1 delta; WIP 1; WIP1; AV338790; PPM1D; PPM1D_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 信号转导 细胞凋亡 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 67kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PPM1D |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: PPM1D (Wip) is a serine/threonine phosphatase implicated in cell cycle control, spermatogenesis, and lymphoid cell function. The predicted 605-amino acid PPM1D protein contains 2 putative nuclear localization signals and 3 regions conserved in serine/threonine PP2C phosphatases, as well as characteristics of a type 2C phosphatase, including magnesium dependence and relative insensitivity to okadaic acid. PPM1D expression is induced in response to ionizing radiation in a p53-dependent manner. The accumulation of PPM1D mRNA following ionizing radiation is rapid and transient, and PPM1D protein is localized to the nucleus. PPM1D may contribute to growth inhibitory pathways activated in response to DNA damage in a p53-dependent manner. PPM1D inhibits phosphorylation of the p38 mitogen-activated (MAP)kinase protein. Through p38 MAPK, PPM1D modulates the CDKN2A tumor-suppressor locus. This gene is located in a chromosomal region known to be amplified in breast cancer, (located at 17q22-q23), is amplified in human breast tumor cell lines and in approximately 11% of primary breast tumors, and appears to lead to cell transformation by abrogating p53 tumor suppressor activity. Inactivation of the p38 MAPK through PPM1D overexpression resulting from PPM1D amplification may contributes to the development of human cancers by suppressing p53 activation. PPM1D null mice have increased susceptibility to pathogens and reduced male fertility and longevity. Function: Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Subunit: Interacts with CHEK1 and CHEK2; dephosphorylates them. Similarity: Belongs to the PP2C family. Contains 1 PP2C-like domain. Database links: Entrez Gene: 8493 Human Entrez Gene: 53892 Mouse Entrez Gene: 380383 Xenopus laevis Entrez Gene: 394065 Zebrafish Omim: 605100 Human SwissProt: O15297 Human SwissProt: Q5RI25 Mouse SwissProt: Q9QZ67 Mouse Unigene: 286073 Human Unigene: 45609 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Wip1是一种镁依赖性的丝氨酸/苏氨酸蛋白磷酸酶,属于蛋白磷酸酶2C家族。Wip1通过p53诱导在癌症的发生、发展中起到重要作用,在多种人类癌细胞中都已发现Wip1的大量扩增和过量存在 |
PPM1D（WIPP）是一种丝氨酸/苏氨酸磷酸酶，参与细胞周期调控、精子发生和淋巴细胞功能。预测的605个氨基酸PPM1D蛋白包含2个假定的核素定位信号和3个在丝氨酸/苏氨酸P2C磷酸酶中保守的区域，以及2C磷酸酶的特征，包括镁依赖性和对冈田酸的相对不敏感。PPM1D的表达是在电离辐射中以p53依赖的方式诱导的。PPM1D mRNA在电离辐射后的积累是快速和短暂的，并且PPM1D蛋白定位于细胞核。PPM1D可能有助于以p53依赖的方式响应于DNA损伤而激活的生长抑制通路。PPM1D抑制p38丝裂原活化（MAP）激酶蛋白的磷酸化。通过p38 MAPK，PPM1D调节CDKN2A肿瘤抑制基因位点。该基因位于乳腺癌已知的染色体区域（位于17q22-q23），在人乳腺肿瘤细胞系和大约11%的原发性乳腺肿瘤中扩增，并通过放弃p53肿瘤抑制活性而导致细胞转化。通过PPM1D扩增导致的PPM1D过表达使p38 MAPK失活可能通过抑制p53活化而促进人类癌症的发展。PPM1D空白小鼠增加了对病原体的敏感性，降低了男性生育能力和寿命。