FITC标记的一氧化氮合成酶-2（诱导型）抗体
产品名称： FITC标记的一氧化氮合成酶-2（诱导型）抗体
英文名称： Anti-iNOS/FITC
产品编号： HZ-0162R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： Flow-Cyt=1:50-200 IF=1:50-200
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Rabbit Anti-iNOS/FITC Conjugated antibody
FITC标记的一氧化氮合成酶-2（诱导型）抗体
| 英文名称 | Anti-iNOS/FITC |
| 中文名称 | FITC标记的一氧化氮合成酶-2（诱导型）抗体 |
| 别 名 | i NOS; Nitric Oxide Synthase, Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible macrophage; Nitric oxide synthase 2A (inducible hepatocytes); Nitric oxide synthase inducible; NOS 2; NOS 2A; NOS; Nos II; NOS type II; Nos2; NOS2A; NOS2_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 神经生物学 信号转导 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, |
| 产品应用 | Flow-Cyt=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 130kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NOS-2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2). Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Subunit: Homodimer. Binds SLC9A3R1. Tissue Specificity: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Similarity: Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. Database links: Entrez Gene: 4843 Human Entrez Gene: 18126 Mouse Entrez Gene: 24599 Rat Omim: 163730 Human SwissProt: P35228 Human SwissProt: P29477 Mouse SwissProt: Q06518 Rat Unigene: 709191 Human Unigene: 2893 Mouse Unigene: 10400 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 合成与降解（Synthesis and Degradation） 催化生物体内一氧化氮（NO）生成的酶。分神经型一氧化氮合成的酶（nNOS or NOS-1）、诱导型一氧化氮合成的酶（iNOS or NOS-2）、内皮型一氧化氮合成的酶（eNOS or NOS-3） |
一氧化氮（NO）是一种无机的、气态的自由基，在细胞之间携带多种信息。血管舒张、神经传递和细胞毒性均可通过细胞对NO的反应而增强。NO的产生由一氧化氮合酶（NOS）家族的成员介导。NOS催化L-精氨酸氧化产生L-瓜氨酸和NO。已克隆出脑或神经元型NOS(b或nNOS，Ⅰ型)和内皮细胞型NOS(eNOS，Ⅲ型)和一种诱导型异构体(iNOS，Ⅱ型)。所有NOS亚型都含有钙调素、烟酰胺腺嘌呤二核苷酸磷酸盐（NADPH）、黄素腺嘌呤二核苷酸（FAD）和黄素单核苷酸（FMN）结合结构域。一氧化氮合酶在肝脏、巨噬细胞、肝细胞、滑膜细胞、受刺激的胶质细胞和平滑肌细胞中表达。细胞因子如干扰素-γ(IFN)、肿瘤坏死因子(TNF)、白细胞介素-1和-2、脂多糖(LPS)引起iNOS mRNA、蛋白质和活性水平的增加。蛋白激酶C刺激剂对iNOS活性表现出相同的作用。在细胞因子诱导后，iNOS表现出延迟的活性反应，随后在长期内NO生成显著增加。人iNOS由钙/钙调蛋白调节（与小鼠NOS2相反）。